Primer Tm
One melting temperature from the DNA sequence.
live · calculated locally
TM
Tm (°C) = 1000 ΔH / (ΔS + 1.987 ln C) − 273.15
Method notes
ΔS salt term = 0.368 × (N − 1) × ln[Na+]
Na+ is mol/L (entered mM × 10⁻³). Blank salt uses 50 mM.
C = 250 × 10⁻⁹ mol/L. Complement neglected.
ΔH is kcal/mol; ×1000 converts it to cal/mol.
nearest-neighbor
SantaLucia 1998 unified parameters. No symmetry term.
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