Lab Calculators
Molarity, dilution, primers, PCR setup, oligo resuspension, cloning helpers, and short lab how-tos. Every tool runs in your browser.
Tools
9 tools- CH 1 · SOLUTIONS
Molarity
Mass, molecular weight and volume to concentration.
out: mol/L · mmol/LOpen → - CH 3 · SOLUTIONS
Dilution Factor
DF from C1/C2 or volumes, serial total DF, and hemocytometer cell counting.
out: 1:N · N×Open → - CH 5 · SOLUTIONS
C1V1
One dilution step. Solve any one of C1, V1, C2, or V2.
warns below your pipette minimumout: C1V1 = C2V2Open → - CH 2 · SOLUTIONS
Serial Dilution
Concentrations, transfer volume, and diluent volume for every step.
warns below your pipette minimumout: c₁…cₙ · µL · diluentOpen → - CH 4 · PCR & PRIMERS
Primer Tm
Nearest-neighbor melting temperature.
out: °COpen → - CH 9 · PCR & PRIMERS
Oligo Resuspension
Oligo resuspension & concentration: nmol or OD260/µg to stock µM, plus working dilution.
out: µL stock · µL diluentOpen → - CH 8 · PCR & PRIMERS
PCR Master Mix
Per-reaction volumes and a master mix total.
warns below your pipette minimumout: µL / rxn · total µLOpen → - CH 6 · CLONING
Ligation
Insert mass for an insert:vector molar ratio.
out: ng · pmolOpen → - CH 7 · CLONING
DNA Copy Number
Copies from dsDNA mass and length.
out: copies · ng · pmolOpen →
Guides
short how-tosGuide
How to calculate dilution factor
Dilution factor formula DF = Vfinal ÷ aliquot = C1 ÷ C2, with lab and cell-counting examples.
out: examples · calculator links
Open →
Guide
How to make 70% ethanol
C1V1 volumes for 70% ethanol from 100%, 95%, or 200 proof. Add water to the final volume.
out: examples · calculator links
Open →